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Phenol-chloroform extraction followed by ethanol precipitation is a well-established method of purification. It involves mixing an aqueous nucleic acid. First time using phenol chloroform isoamyl alcohol () for DNA purification. Product is purchased and not prepared in the lab. Phenol/chloroform extraction is a common technique used to separate and purify DNA, RNA, and protein from a biological specimen, such as a virus preparation.

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Attach the experimental protocol(s) for phenol chloroform extractions. POTENTIAL HAZARDS. Principal Materials Used, Corrosive, Irritant, Sensitizer. Extracting DNA Using Phenol-Chloroform. Reagents Needed. 1. Phenol/chloroform/isoamyl alcohol (PCI) solution () DNase (RNase- and Protease-Free -. Phenol-chloroform extraction is a liquid-liquid extraction technique in biochemistry and molecular biology for purifying DNA contaminated by histones and.

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UltraPure Phenol:Chloroform:Isoamyl Alcohol (, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform. Mix your sample with 1 volume of Tris-saturated phenol and 1 volume of chloroform. Centrifuge at 10, rpm for 5 min at room temperature. 2. Transfer the upper. PHENOL:CHLOROFORM pH /, Biotechnology Grade, ML, (A) - Products QBIC (Quality Biological). (Amresco ML) PHENOL:CHLOROFORM.